High-Resolution Imaging of Cellular Proteins Methods and Protocols
High-Resolution Imaging of Cellular Proteins Methods and Protocols by Steven D. Schwartzbach is a comprehensive resource published by Springer New York on June 9, 2018. This edition spans 366 pages and is presented in English. The book focuses on advanced methodologies for imaging proteins and investigating their functions through light and electron microscopy, offering a detailed exploration of the techniques involved.
Readers will find a structured approach to three main toolboxes that encompass the development of molecular and immunological probes, high-resolution fluorescence microscopy, and applications for transmission and scanning electron microscopy. Each chapter provides an introduction to its topic, lists necessary materials and reagents, and includes step-by-step laboratory protocols designed for reproducibility. The book aims to facilitate successful outcomes in the study of biochemistry and related fields, making it a valuable addition for those in the biological sciences and medical professions.
Official synopsis Publisher
This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.
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