Hedgehog Signaling Methods and Protocols
Hedgehog Signaling Methods and Protocols by Xiaochun Li, published by Springer US on September 28, 2022, is a comprehensive resource that delves into the technical advancements in understanding the molecular mechanisms of hedgehog (HH) signaling. This edition spans 244 pages and is presented in English, offering a detailed examination of the biophysical and cell biological approaches that have propelled research in this area, particularly highlighting the role of sterols in HH signaling.
Readers will find a thorough exploration of key protein components involved in the HH pathway, including the signaling ligand HH, the secretion regulator Dispatched, the receptor Patched, the signal transducer Smoothened, and the transcription factor GLI. The book is structured in the well-regarded Methods in Molecular Biology series format, featuring introductions to each topic, lists of necessary materials and reagents, and step-by-step protocols that are designed to be readily reproducible. This authoritative guide serves as a practical reference for researchers in both clinical and basic science, facilitating a deeper understanding of HH signal transduction through various scientific approaches.
Official synopsis Publisher
This detailed book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, indicating the important role of sterols in this pathway. Within its pages, the volume examines techniques involving several key protein components in this pathway, including HH, the signaling ligand; Dispatched, a HH secretion regulator; Patched, the receptor of HH; Smoothened, the signal transducer used to transduce the HH signal across the plasma membrane; and GLI, the transcription factor to turn on HH target genes. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Hedgehog Signaling: Methods and Protocols provides researchers in clinical and basic science with a fresh and reliable reference for analyzing HH signal transduction through cell biological, biochemical, and biophysical approaches.
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