Bacterial Regulatory RNA Methods and Protocols
Bacterial Regulatory RNA Methods and Protocols by Véronique Arluison, published by Springer New York on February 27, 2018, is a comprehensive resource spanning 395 pages. This volume focuses on essential methods for studying prokaryotic non-coding RNAs and their associated proteins, providing a structured approach to the biology of this field.
Readers will find detailed chapters that cover various aspects, including the identification of non-coding RNAs, their differential expression, and characterization of their structure and function. The book also explores interactions with RNA binding proteins and potential applications in synthetic biology. Each chapter includes introductions, lists of necessary materials, step-by-step laboratory protocols, and troubleshooting tips, making it a practical guide for scientists engaged in sRNA biology.
Official synopsis Publisher
This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements in the rapidly emerging field of synthetic biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols serves as a guidebook for scientists working toward the development of new tools and proceduresfor the vital field of sRNA biology.
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