Nuclear Bodies and Noncoding RNAs Methods and Protocols
Nuclear Bodies and Noncoding RNAs: Methods and Protocols by Shinichi Nakagawa, published by Springer New York on January 2, 2015, is a comprehensive volume consisting of 351 pages. This edition presents a detailed examination of cytological, biochemical, and molecular biological methods aimed at identifying and analyzing the functions of nuclear bodies, with a particular focus on long non-coding RNAs.
Readers will find a structured approach to understanding how specific long non-protein-coding RNAs accumulate in nuclear bodies and influence their functions. The chapters include introductions to each topic, lists of necessary materials and reagents, and step-by-step laboratory protocols designed for reproducibility. This book serves as a practical guide for those interested in the intricate regulation of gene expression, making it a valuable resource in the fields of medical genetics and clinical medicine.
Official synopsis Publisher
This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nuclear Bodies and Noncoding RNAs: Methods and Protocols serves as a guideline for further study into the sophisticated regulation of gene expression.
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