Inositol Phosphates and Lipids Methods and Protocols
Inositol Phosphates and Lipids: Methods and Protocols by Christopher J. Barker, published by Humana Press on July 1, 2012, is a comprehensive resource that explores the methodologies for measuring inositol lipids and phosphates. This edition, consisting of 230 pages, presents a detailed overview of over 30 unique isomeric forms of inositol, highlighting their diverse regulatory roles in cellular functions. The book introduces both foundational techniques and recent advancements, including RNA-silencing and real-time measurement methods using fluorescently labeled PH-domains.
Readers will find a structured approach to laboratory protocols, with each chapter offering introductions to key topics, necessary materials, and step-by-step instructions designed for reproducibility. The text also includes troubleshooting tips to help avoid common pitfalls in the experimental process. Covering essential subjects such as molecular biology and cell biology, this guide serves as a valuable reference for researchers interested in the intricate roles of inositol phosphates and lipids in life sciences.
Official synopsis Publisher
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.
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