Biological Low-Voltage Scanning Electron Microscopy
Biological Low-Voltage Scanning Electron Microscopy by James Pawley, published by Springer New York on September 13, 2014, is a detailed exploration of the advancements in scanning electron microscopy (SEM) as a biological imaging technique. This edition, comprising 317 pages, delves into the significant improvements in instrumentation and specimen preparation that have enhanced the capabilities of field-emission scanning electron microscopy (FESEM), offering superior spatial resolution and reduced sample damage.
Readers will find a thorough examination of the theory and practical applications of FESEM in biological contexts. The book provides an in-depth discussion of the instrumentation, applications, and protocols necessary for operating these advanced microscopes effectively. It aims to equip readers with the knowledge required to achieve high-quality imaging in various fields, including molecular biology and microbiology, making it a valuable resource for those interested in the life sciences.
Official synopsis Publisher
Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM.
Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.
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