Protein NMR Methods and Protocols

“Protein NMR Methods and Protocols” by Ranajeet Ghose is a comprehensive resource published by Springer New York on November 19, 2017. This 1st edition volume, comprising 446 pages, explores advanced applications of nuclear magnetic resonance (NMR) spectroscopy in the study of protein structure, dynamics, and interactions. The book presents a detailed overview of data acquisition and processing, as well as the determination of structures and dynamics across a range of biological entities, from individual proteins to large macromolecular complexes.
Readers will find a wealth of information in this volume, which includes laboratory protocols and troubleshooting tips tailored for NMR spectroscopists at various expertise levels. The chapters delve into the intricacies of solid-state and solution NMR techniques, emphasizing their potential in studying proteins and protein complexes of significant biological relevance. This edition serves as a practical guide for those engaged in medical and clinical research, particularly in the fields of radiology and diagnostic imaging.
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This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise.
Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.
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