Promoter Associated RNA Methods and Protocols

Promoter Associated RNA Methods and Protocols by Sara Napoli is a comprehensive resource published by Springer New York on March 28, 2017. This 1st edition, consisting of 287 pages, is presented in English and focuses on the emerging field of promoter-associated RNAs (paRNAs). The volume is structured into four sections that explore genome-wide approaches, techniques for characterizing paRNA structural features, selection methods for paRNAs, and their therapeutic potential.

Readers will find detailed chapters that provide introductions to each topic, necessary materials and reagents, and step-by-step laboratory protocols designed for reproducibility. The book emphasizes the application of siRNAs directed against paRNAs to modulate the transcription of key genes influenced by these regulatory molecules. By presenting authoritative methodologies, this work aims to enhance understanding of paRNAs as a new class of regulatory molecules and their potential as tools for fine transcriptional tuning in the fields of medical genetics and clinical medicine.