Integrated Methods in Protein Biochemistry: Part B
Integrated Methods in Protein Biochemistry: Part B by Arun K. Shukla, published by Elsevier Science on January 18, 2023, is a comprehensive volume within the Methods in Enzymology series, consisting of 420 pages. This edition focuses on recent advancements in protein biochemistry, presenting a range of topics that include the precise modification of native proteins, purification and analysis of bioconjugates, and methods for monitoring receptor tyrosine kinases.
Readers will find detailed chapters that explore various techniques and protocols, such as spectroscopic analysis of cysteine dioxygenase and MS-based analysis of viral spike N-glycoforms. The book also delves into the characterization of GPCR signaling complexes and the structural analysis of lytic polysaccharide monooxygenases, among other subjects. This edition serves as a valuable resource for those interested in molecular biology and biochemistry, providing insights into both established and innovative methodologies in the field.
Official synopsis Publisher
Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ?? subunit and PLC-? enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more.
Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.
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