Enzyme Assays: A Practical Approach (The Practical Approach Series, 90)
“Enzyme Assays: A Practical Approach” by Robert Eisenthal, published by Oxford University Press on April 23, 1992, is a comprehensive resource spanning 376 pages. This book provides an in-depth examination of enzyme assays, which are essential procedures in biochemistry used to estimate enzyme quantities, track purification processes, and determine kinetic parameters. It details the design and execution of various enzyme assays, presenting both general principles and specific examples to guide researchers.
Readers will find a wealth of information on experimental protocols, including photometric, electrochemical, radiochemical, and HPLC techniques, as well as methods for assessing enzyme activity post-gel electrophoresis. The book outlines the theoretical foundations of each method, instrumentation details, sensitivity considerations, and potential sources of error. Additionally, it addresses enzyme extraction techniques, statistical analysis of kinetic data, and offers insights into buffers and protein estimation methods. This work serves as a valuable reference for biochemists and researchers across the life sciences.
Official synopsis Publisher
Enzyme assays are one of the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific examples. Chapters contain experimental protocols, including photomeric, electrochemical, radiochemical, and HPLC techniques, along with methods for determining enzyme activity after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also discussed are the techniques and problems associated with enzyme extraction, statistical analysis of kinetic data, and a chapter examining buffers and methods of protein estimation. This work will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
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