Cardiac Gene Therapy Methods and Protocols
Cardiac Gene Therapy Methods and Protocols by Kiyotake Ishikawa, published by Springer US on August 31, 2022, is the second edition of a comprehensive volume that delves into advanced techniques in cardiac gene therapy. Spanning 347 pages, this edition expands on the previous one, offering updated discussions on gene suppression, editing, and reprogramming, as well as various delivery methods and patient screening for cardiac gene therapy.
Readers will find a structured approach to the subject, with chapters that include introductions, necessary materials, and step-by-step protocols designed for reproducibility in laboratory settings. This edition emphasizes troubleshooting and avoiding common pitfalls, making it a practical resource for researchers in the fields of cardiology and genetics. The book aims to facilitate advancements in gene therapy research and its clinical applications, contributing to the ongoing developments in medical science.
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This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field.
Chapter “Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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