Biotechnology: A Laboratory Course
Biotechnology: A Laboratory Course by Jeffrey M. Becker is a comprehensive resource published by Elsevier S & T in 1996. This second edition consists of 261 pages and is presented in English. The text features a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques, designed to guide readers from basic techniques to more advanced applications. The course utilizes two organisms, Saccharomyces cerevisiae and Escherichia coli, along with a single plasmid and enzyme, providing a foundation that is applicable to various biological systems.
Readers will find updated exercises and appendixes that enhance the learning experience, including new content on polymerase chain reaction, beta-galactosidase detection, and Western blotting. The edition introduces procedures for large-scale plasmid isolation, yeast transformation, and DNA quantitation, along with a new appendix detailing access to biological information on the Internet. This text serves as a valuable aid for establishing or instructing introductory courses in biological sciences, making it suitable for those interested in biotechnology, genetics, and molecular biology.
Official synopsis Publisher
The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences.
Key Features of this new edition:
* All exercises and appendixes have been updated
* Includes new exercises on
* Polymerase chain reaction
* Beta-Galactosidase detection in yeast colonies
* Western blotting
* New procedures introduced for
* Large-scale plasmid isolation
* Yeast transformation
* DNA quantitation
* New appendixes added, one of which provides details on accessing biological information sites on the Internet (World Wide Web)
* Use of non-radioactive materials and easy access to microbial cultures
* Laboratory exercises student tested for seven years
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