Basophils and Mast Cells Methods and Protocols
Basophils and Mast Cells: Methods and Protocols by Bernhard F. Gibbs is a comprehensive resource published by Springer US on August 7, 2020. This second edition spans 380 pages and is presented in English, offering updated and new chapters that enhance the foundational knowledge established in the first edition. The book delves into the basic biology of basophils and includes detailed methodologies for obtaining and culturing these cells, as well as exploring their roles in various asthma phenotypes and diagnostic applications.
Readers will find a structured approach to the subject, with each chapter featuring introductions, lists of necessary materials and reagents, and step-by-step laboratory protocols designed for reproducibility. The text also provides insights into gene expression patterns in basophils and discusses knockout and disease models. This edition is part of the Methods in Molecular Biology series, ensuring that it meets the needs of researchers in the fields of medical science, immunology, and cell biology.
Official synopsis Publisher
This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
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