Microbial Steroids Methods and Protocols

Microbial Steroids Methods and Protocols by José-Luis Barredo, published by Springer New York on July 15, 2017, is a comprehensive guide that offers practical experimental laboratory protocols for various steroid bioconversions. This 1st edition, comprising 373 pages, presents a structured approach to topics such as bioconversions, chemical synthesis pathways, strain characterization, and steroid hydroxylations, making it a valuable resource for professionals in the field.
Readers will find detailed chapters that include introductions to each topic, lists of necessary materials and reagents, and step-by-step protocols designed for reproducibility. The book also provides insights into biocatalysis and downstream processes for purifying steroid intermediates. With its focus on life sciences and biochemistry, Microbial Steroids Methods and Protocols serves as an essential reference for laboratory and industrial professionals, as well as graduate students in biotechnology, microbiology, genetics, and molecular biology.
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This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.
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