Practical Molecular Virology

Practical Molecular Virology by Mary K. Collins, published by Humana Press in October 1991, is a comprehensive resource that presents a collection of methods for gene transfer and expression utilizing various recombinant viral vectors. This edition, comprising 330 pages, is designed to be accessible for both novices and experienced researchers, featuring techniques laid out in an easy-to-follow format.
Readers will find detailed chapters covering the life cycles of specific retroviruses, the construction of recombinant vectors, and various PCR techniques. The book also explores poliovirus and herpesvirus vectors, syncytial assays, cell lineage studies, and the use of baculovirus and adenovirus vectors. With a focus on recombinant retroviral methods and their applications, Practical Molecular Virology serves as a valuable reference for those engaged in biological and biomedical research.
Official synopsis Publisher
Mary K. L. Collins has assembled in Practical Molecular Virology a vanguard collection of readily repeatable methods for gene transfer and expression using a variety of recombinant viral vectors. In keeping with the established tradition of the series, each technique is presented in an easy-to-follow format designed to work for the novice as well as the seasoned expert. Chapters cover: • life cycles of specific retroviruses and how recombinant vectors are constructed • PCR techniques • poliovirus vectors • herpesvirus vectors • syncytial assays • cell lineage studies • baculovirus and adenovirus vectors • SV40 and EBV vectors • viruses in gene transfer to eukaryotic cells The wealth of material devoted to recombinant retroviral methods and their applications make Practical Molecular Virology and extremely timely volume, one that will find widespread use throughout biological and biomedical research.
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