Microbial Toxins Methods and Protocols
Microbial Toxins: Methods and Protocols by Otto Holst is a comprehensive resource published by Springer New York on May 6, 2017. This second edition expands on previous editions, offering detailed protocols focused on (cyano) bacterial and mold fungus toxins, with a particular emphasis on Gram-negative endotoxins, such as lipopolysaccharides. The book includes a variety of techniques, including methods for detecting cholera toxin and Listeria in food, as well as protocols for endotoxin removal from bacterial lysates.
Readers will find a structured approach to laboratory techniques, with each chapter providing introductions, necessary materials, and step-by-step protocols designed for reproducibility. The content is tailored for scientists and researchers in the fields of microbiology and life sciences, making it a practical tool for those looking to enhance their understanding of microbial toxins. With 201 pages of focused methodologies, this edition serves as a valuable addition to the Methods in Molecular Biology series.
Official synopsis Publisher
This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.
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