Zebrafish Methods and Protocols

Zebrafish Methods and Protocols by James F. Amatruda is a comprehensive resource published by Springer US on September 6, 2023. This third edition spans 360 pages and is presented in English, offering an updated exploration of zebrafish as a vital model for developmental and disease biology research. The book introduces new tools, models, and analytic insights, beginning with methods for using zebrafish to model various human diseases.
Readers will find detailed chapters that illuminate the zebrafish’s role in studying the vertebrate nervous system, stem cell biology, and genetics. Each chapter includes introductions to the topics, lists of necessary materials and reagents, and step-by-step laboratory protocols that are readily reproducible. This edition serves as an authoritative guide for researchers in the fields of life sciences and biology, focusing on methodologies that enhance understanding of regeneration and disease modeling using zebrafish and medaka.
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This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.
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