Baculovirus and Insect Cell Expression Protocols
Baculovirus and Insect Cell Expression Protocols by David W. Murhammer is a comprehensive guide published by Springer New York on March 31, 2018. This third edition expands upon previous versions with updated methods and protocols, providing a detailed overview of techniques related to cell growth, baculovirus infection, and cell metabolism. The book includes step-by-step procedures for isolating new cell lines, developing serum-free media, and maintaining insect cell lines, along with insights into recombinant protein production and the use of baculoviruses in various applications.
Readers will find a wealth of information structured in the familiar Methods in Molecular Biology series format, which features introductions to each topic, lists of necessary materials, and reproducible laboratory protocols. The text covers advanced topics such as the production and characterization of baculoviruses, the use of green fluorescent protein, and the application of baculovirus/insect cell systems in studying apoptosis. With 508 pages of authoritative content, this edition aims to assist users in successfully executing the described tasks while encouraging the development of innovative techniques in the fields of microbiology and virology.
Official synopsis Publisher
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
Publisher
Topics
FAQ
What is “Baculovirus and Insect Cell Expression Protocols” about?
Who is the author of “Baculovirus and Insect Cell Expression Protocols”?
When was “Baculovirus and Insect Cell Expression Protocols” published?
What is the ISBN for “Baculovirus and Insect Cell Expression Protocols”?
What are the book details (language, pages, edition)?
