HPLC and UHPLC for Practicing Scientists
HPLC and UHPLC for Practicing Scientists by Michael W. Dong is a comprehensive reference guide published by John Wiley & Sons on July 23, 2019. This second edition spans 416 pages and is written in English, focusing on the fundamentals and latest developments in high-performance liquid chromatography (HPLC) and ultra-high-pressure liquid chromatography (UHPLC). The book serves as a resource for both novice and experienced practitioners in the pharmaceutical and biotechnology industries, providing essential information on best practices and modern trends.
Readers will find detailed discussions on HPLC and UHPLC fundamentals, including theory, applications, and instrumentation. This edition introduces three new chapters covering UHPLC concepts, liquid chromatography/mass spectrometry (LC/MS), and the analysis of recombinant biologics, particularly monoclonal antibodies. Each chapter has been revised, with significant updates to topics such as method development and regulatory compliance. The book also includes problem and answer sections at the end of each chapter, making it a valuable tool for graduate students and practicing scientists seeking to enhance their understanding of these analytical techniques.
Official synopsis Publisher
A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries
Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner.
HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter.
- Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references
- Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics
- Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC
- Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects
- Includes end-of-chapter quizzes as assessment and learning aids
- Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries
Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.
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