Difference Gel Electrophoresis Methods and Protocols
Difference Gel Electrophoresis Methods and Protocols by Kay Ohlendieck is a comprehensive resource published by Springer US on November 16, 2023. This second edition spans 485 pages and is presented in English. It offers an updated exploration of gel-based proteomics, focusing on differential protein labeling and two-dimensional gel electrophoresis, along with optimized workflows for protein identification using advanced mass spectrometry.
Readers will find detailed chapters that include introductions to key topics, necessary materials and reagents, and step-by-step laboratory protocols designed for reproducibility. The book also addresses troubleshooting techniques and common pitfalls, making it a valuable guide for those engaged in biological research, pathobiology, and biomarker discovery. With its authoritative approach, this edition aims to facilitate successful outcomes in the study of biochemistry and biophysics.
Official synopsis Publisher
The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
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