Polymerase Chain Reaction Theory and Technology
“Polymerase Chain Reaction Theory and Technology” by Mark A. Behlke, published by Caister Academic Press in 2019, is a comprehensive resource that delves into the intricacies of polymerase chain reaction (PCR) technology. Spanning 262 pages, this book serves as a manual for researchers and practitioners across various scientific disciplines, including microbiology and molecular biology. It presents a detailed examination of PCR principles, instrumentation, and methodologies, making it a valuable addition to any laboratory or academic library.
Readers will find a thorough exploration of essential topics such as fluorescent chemistries, quantification strategies, and the extraction and purification of nucleic acids. The book also addresses critical aspects like sample preparation, validation controls, and the standardization of methods, alongside the MIQE guidelines and mRNA expression. This compilation of expert review articles offers insights into both the theoretical and practical applications of PCR, catering to those interested in advancing their understanding and use of this vital laboratory technique.
Official synopsis Publisher
The polymerase chain reaction (PCR) is a powerful research tool used in many scientific disciplines. It is also used for detection and testing in areas such as food microbiology, environmental microbiology, biotechnology, industrial microbiology, veterinary and medical diagnostics. This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.
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